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XU LABORATORY
INSTITUTE FOR CELL ENGINEERING

DEPARTMENT OF NEUROLOGY | JOHNS HOPKINS SCHOOL OF MEDICINE
Human iPSC for Disease Modelling & Cellular Therapy

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All-In-One human brain miniature
Microglia actively monitor the environment within brain organoids that have vascular endothelia & pericytes experimentally introduced.


CD31/PDGFRβ
(endothelia & pericytes)
Enriched Neural Stem Cells
FOXG1/Nestin neural stem from hiPSC
FOXG1+ neurospheres


Human Cortical Neurons
Layer specific neurons (I - VI)
Balanced excitatory & inhibitory neurons

Midbrain dopaminergic neurons
TH/MAP2
Enriched Astrocytes GFAP & DAPI
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Crosstalk between neurons and astrocytes
MAP2
GFAP
MAP2/GFAP

EGFP hiPSC
&
RFP hiPSC

EGFP hiPSC

RFP hiPSC

Mixed EGFP & RFP hiPSC suspension culture
Microglia precursor cells derived from Yolk Sac
(Differentiated from EGFP & RFP hiPSC)

Vascular Endothelia and Pericytes
Vasculature like meshwork with cardiac microtissues from hiPSC



Cardiac Tissue
3D organotypic cardiac microtissues from hiPSC

Bronchiole (small airway) like structure from hiPSC

Microfluidic Device

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