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XU LABORATORY
INSTITUTE FOR CELL ENGINEERING
DEPARTMENT OF NEUROLOGY | JOHNS HOPKINS SCHOOL OF MEDICINE
3D-Organoid
Bridging complexity: from stem cells to living tissue microenvironments.
Schematic of immune-Vascularized Brain Organoids (iVBOs)
forebrain organoid-based multicellular assembled cerebral organoid (FORMA-CO)
Immune-Vascularized Brain Organoids (iVBO aka FORMA-CO) (link)
Microglia actively monitor the environment within brain organoids
CD31/PDGFRβ
(endothelia & pericytes)
Microglia
iVBO organoid
iVBO (sectioned then immunostained)
Human iPSC for Disease Modeling & Cellular Therapy
Created with BioRender.com
Neural lineages
Multipotent neural stem cells
Enriched Neural Stem Cells
FOXG1/NESTIN
(neural stem cell from hiPSC)
FOXG1 Organoids
Human Cortical Neurons
Layer specific neurons (I - VI)
Balanced excitatory : inhibitory neurons (E/I)
E/I: 2:1 in most cortical areas; rodents have higher E:I ratios (e.g., 5:1)
Created with BioRender.com
Human iPSC-derived Cortical Neurons
Midbrain dopaminergic neurons
TH/MAP2
_JPG.jpg)
Enriched Astrocytes GFAP & DAPI
Crosstalk between neurons and astrocytes
MAP2
GFAP
MAP2/GFAP
EGFP & RFP hiPSC lines
EGFP hiPSC
RFP hiPSC
Mixed EGFP & RFP hiPSC suspension culture
Microglia precursor cells of iPSC-Yolk Sac
(Differentiated from EGFP & RFP hiPSC)
Vascular Endothelia and Pericytes
(Vasculature like meshwork with cardiac microtissues from hiPSC)
Cardiac Tissue
(3D organotypic cardiac microtissues from hiPSC)
Bronchiole (small airway) like structure from hiPSC
iPSC-Neurons in Microfluidic Device
iNEST for Neocortical Function Restoration (stroke or TBI)
GFP-iNEST
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